I could not, for the life of me, get a good concentration of RNA yesterday. And it’s driving me crazy.
I know it’s not my technique, because I tested it with some old tissue samples in the -80, and got half a microgram per microliter. But with the real samples, I got between 9 and 60 nanograms.
I know it’s not the Nanodrop machine, because I tested them on two different ones.
I know it’s not due to my scraping the cells badly, because I tested samples that PhD scraped when she was showing me how to do it.
It’s just maddening. It annoyed me all last night, and then I had nightmares about my boss yelling at me and telling me my work was horrible and I was going to irreparably damage PhD’s thesis project and keep her from her degree.
So excuse me for a day or so while I stew.